A Basic Guide to Real Time PCR in Microbial Diagnostics | 台灣精品獎-歷屆得獎名單
![A Basic Guide to Real Time PCR in Microbial Diagnostics](https://i.imgur.com/DERULla.jpg)
由PKralik著作·2017·被引用518次—RealtimePCR(quantitativePCR,qPCR)isnowawell-establishedmethodforthedetection,quantification,andtypingofdifferentmicrobial ...
![A Basic Guide to Real Time PCR in Microbial Diagnostics](https://i.imgur.com/DERULla.jpg)
The phrase “Polymerase chain reaction” (PCR) was first used more than 30 years ago in a paper describing a novel enzymatic amplification of DNA (Saiki et al., 1985[1]). The first applications of PCR were rather unpractical due to the usage of thermolabile Klenow fragment for amplification, which needed to be added to the reaction after each denaturation step. The crucial innovation which enabled routine usage of PCR was utilization of thermostable polymerase from Thermus aquaticus (Saiki et al., 1988[2]). This improvement, together with the availability of PCR cyclers and chemical components, led to the worldwide recognition of PCR as the tool of choice for the specific enzymatic amplification of DNA in vitro. It must be noted that the general concept of PCR, which includes primers, DNA polymerase, nucleotides, specific ions, and DNA template, and consisting of cycles that ...
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A Basic Guide to Real Time PCR in Microbial Diagnostics | 台灣精品獎-歷屆得獎名單
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![](https://i.imgur.com/DERULla.jpg)
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