A Step-by | 台灣精品獎-歷屆得獎名單
2021年5月6日—Thefirststepindesigningprimersistogetthenucleotidesequenceofyourgeneofinterest.GotothePubmedgenedatabaseandsearchfor ...
Quantitative PCR (qPCR) primer design is a critical step when setting up your qPCR or reverse transcription-qPCR assay (RT-qPCR)[1] for gene expression analysis. qPCR primers that anneal poorly or that anneal to more than one sequence during amplification can significantly impact the quality and reliability of your results.
Also, if you are performing a one-step reverse transcription PCR (RT-qPCR), the reverse transcriptase[2] will use the reverse primer to prime the transcription reaction. In this scenario, a poor primer would result in both inefficient reverse transcription and inefficient amplification – a lose–lose situation.
Considering the above, it’s well worth spending the time necessary to design good-quality qPCR primers. This article will tell you exactly how to do that!
The good news is that primers are cheap, so you can easily test several differen...
Primer designing tool | 台灣精品獎-歷屆得獎名單
qPCR Assay Design and Optimization | 台灣精品獎-歷屆得獎名單
PrimerQuest | 台灣精品獎-歷屆得獎名單
如何設計優良的qPCR primer | 台灣精品獎-歷屆得獎名單
A Step-by | 台灣精品獎-歷屆得獎名單
qPCR Primer & Probe Design Tool | 台灣精品獎-歷屆得獎名單
108 年度台灣精品獎獲獎產品 TurboCycler 2 核酸聚合酶鏈鎖反應熱循環儀
由「廣普生物科技股份有限公司」生產的TurboCycler2核酸聚合酶鏈鎖反應熱循環儀獲得108年度台灣精品獎,以下為此獎項詳細資...